Simultaneous detection of feline leukaemia antigens and feline immunodeficiency antibodies in blood, serum or plasma.
Ideal both for screening and for clinical differentiation in cats with clinical pictures compatible with both diseases. It detects specific p27 (Leukaemia) and gp40 (Immunodeficiency) antigens: it does not interfere with vaccinations. 2-step technique: savings in time and fewer errors.
Formats available: 5 and 10 tests.
Technical documents only for veterinarians
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Frequently Asked Questions
What is the diagnostic interest of the FeLV-FIV Uranotest?
The qualitative detection of the Feline Leukaemia virus (FeLV) antigen and the Feline Immune Deficiency virus antibody in serum, plasma or whole blood in cats.
Which sample provides the most reliable results: blood, serum or plasma?
Reliability is comparable when you use any of them.
However, when we work with very hemolysed or lipemic blood, it is always preferable to use serum or plasma, since hemolysis and lipemia are factors that interfere with any diagnostic technique, both at the clinic and at the Laboratory.
Can I dispense one drop of blood, serum or plasma directly from the syringe?
No, it is important to use a 10 micro-litre drop of sample, which can only be done with the aid of the disposable precision microcapillary supplied in the kit.
Administering a drop with a volume other than recommended may lead to an erroneous result.
Can I use a tube other than the one included in the kit to collect blood?
Yes. You may use any other tube with anticoagulant treated with heparin, citrate or EDTA or with anticoagulant, if what you wish to do is use serum as a sample. The kit includes tubes with EDTA for your greater comfort and savings, but you may use any other one.
What should I do if I cannot use the blood at the time of its extraction?
You should proceed as if it were for any other laboratory procedure.
The entire blood must be kept refrigerated at between 2ºC and 7ºC for no more than 24 hours. It should never be frozen.
If you are not going to be able to use it before 24 hours, separate the plasma by centrifugation or the serum by sedimentation, and you may keep it at between 2ºC and 7ºC for up to 2 weeks. To preserve it for longer, freezing is recommended.
Can I use blood, serum or plasma immediately after removing it from the refrigerator?
No, as with any other diagnostic procedure, you must wait until it reaches room temperature. If want to speed this process up, hold the tube between your hands for at least 20 minutes. Many veterinarians do not take this circumstance into account, and incorrect temperature is one of the most frequent causes behind false results.
What happens if I forgot to do the reading after 10 minutes?
20 minutes after doing the test, degradation and diffusion of the colours on the strip may occur. This does not happen with all tests, but the recommendation is that the test should always be repeated if the reading was not taken before the indicated time, since this may lead to an erroneous interpretation.
What diagnostic interest does carrying out the FeLV–FIV Uranotest have in screening or prevention campaigns?
Performing a test before putting a cat in the home is mandatory, especially for those coming from an environment without sufficient health guarantees, and periodically, for cats that have easy outside access and frequently escape.
The FeLV-FIV Uranotest has a sensitivity and specificity very close to gold standards (laboratory reference technique) and can detect FeLV-FIV or FIV antibodies early on.
However, performing a test does not exclude pathogenesis of both illnesses, and the different phases of the infection must be taken into account: transitory viremia, persistent viremia or latent infection.
If the cat is in an initial infection stage, FeLV antigens or FIV antibodies are not detected. If we want to completely eliminate the risk of putting sick animals into the home, another test must be performed 90 days after the first one.
Does performing the FeLV Uranotest interfere with vaccines?
No, the Uranotest detects the p27 antigen for the Feline leukaemia virus, which is not normally used for commercial vaccines currently in existence
Can I test kittens of any age?
Yes, kittens may be checked at any age, taking the following factors into account:
In the case of FeLV, there is trans-placenta transmission and through the milk. As such, kittens from positive mothers may also be positive at birth and test negatively later on.
In the case of FIV, up to 12 weeks of life may have passive immunity from an infected mother and give a positive test. However, the virus is transmitted to the litter on only 30% of occasions. If a test is again performed 8-12 weeks later and the result is negative, it is highly improbably that the kitten is infected. On the other hand, if it is positive, we can determine that it is suffering from the infection
How should a positive for FeLV be interpreted with the Uranotest?
The test is the first indispensable step in the diagnostic protocol for Feline leukaemia. A positive for FeLV shows the presence of the viral antigen in the blood, but is not a definitive diagnostic for leukaemia, lymphosarcoma or other illnesses associated with Feline leukaemia. As with any other laboratory procedure, the result must be evaluated along with other clinical and laboratory findings (blood work and other biochemical profiles).
It must be taken into account that cats with transitory viremia may become negative at 4-6 weeks.
How should a negative for FeLV be interpreted with the Uranotest?
A negative to FeLV shows the absence of the viral antigen in the blood at the exact time when it was performed.
This does not exclude infections in an early phase or neutralisation of the infection. For cats that have contacted other infected parties, it is recommended to repeat the test within 8-12 weeks.
How should a positive for FIV be interpreted with the Uranotest?
The test is the first indispensable step in the diagnostic protocol for Feline immunodeficiency. A positive for FIV shows the presence of the viral antigen in blood, but this is not a definitive diagnostic until it is evaluated along with other clinical and laboratory findings.
It must be taken into account that cats with transitory viremia can become negative at 4-6 weeks.
How should a negative for FIV be interpreted with the Uranotest?
A negative for FIV shows a lack of viral antigens in blood at the exact time when it was performed.
In initial phases of infection, no antibodies against FIV are produced. This is why for cats that have had contact with other infected cats, it is recommended to repeat the test within 12 weeks to totally exclude the risk of transmission. Also described is the fact that in the acute phase of the illness, the cat may not have antibodies in circulating blood.
Should I perform an FeLV-FIV determination on any sick animal?
Given the unspecific nature of symptomatology of leukaemia and feline immunodeficiency and the high prevalence, it is advisable to discard its presence by carrying out a test for any unspecific symptomatology pathology process. This is especially advisable for cats with clinical symptoms of oral disease, given the high correlation between oral pathologies and infection by feline retrovirus