Detection of Ehrlichia canis antibodies in whole blood, serum or plasma.
Precise capillary tube incorporated to dispense an exact volume of the sample. Simple 2-step technique: save time and prevent mistakes.
Format: boxes with 5 and 10 tests.
Technical documents only for veterinarians
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Frequently Asked Questions
What is the diagnostic interest of the Ehrlichia Uranotest?
The detection of Ehrlichia canis antibodies in circulating blood.
Can I dispense a drop of blood, serum or plasma directly from the syringe or with the aid of any pipette?
No, it is important to use a 10 micro-litre drop of sample, which you can only do with the aid of the disposable precision microcapillary supplied in the kit.
Unless working with calibrated pipettes, the size of a drop can hugely vary according to the density, the type of disposable pipette used, etc. For diagnostic purposes, a generic drop of blood is not the same as a 10 micro-litre drop of blood.
How does one use the precision microcapillary?
Take the capillary at the middle, between your index finger and thumb. The black band must be at the end that will enter into contact with the sample. Lightly apply pressure, and upon releasing, it will absorb the 10 micro-litres necessary for the test.
Apply pressure again to deposit the drop on the pad.
Can I use a tube other than the one included in the kit to collect blood?
Yes. You may use any other tube with anticoagulant treated with heparin, citrate or EDTA or with anticoagulant, if what you wish to do is use serum as a sample. The kit includes tubes with EDTA for your greater comfort and savings, but you may use any other one.
What should I do if I cannot use the blood at the time of its extraction?
You should proceed as if it were for any other laboratory procedure.
The entire blood must be kept refrigerated at between 2ºC and 7ºC for no more than 24 hours. It should never be frozen.
If you are not going to be able to use it before 24 hours, separate the plasma by centrifugation or the serum by sedimentation, and you may keep it at between 2ºC and 7ºC for up to 72 hours. To preserve it for longer, freezing is recommended.
Can I use blood, serum or plasma immediately after removing it from the refrigerator?
No, as with any other diagnostic procedure, you must wait until it reaches room temperature. If want to speed this process up, hold the tube between your hands. Many veterinarians do not take this circumstance into account, and incorrect temperature is one of the most frequent causes behind false results.
What happens if I forgot to do the reading at 20 minutes as recommended?
Between 20 and 30 minutes, you can read with total reliability.
30 minutes after doing the test, degradation and diffusion of the colours on the strip may occur. This does not happen with all tests, but the recommendation is that the test should always be repeated if the reading was not taken before the indicated time, since this may lead to an erroneous interpretation.
How should I interpret a positive result?
A positive test indicates that there are antibodies against E. canis in circulating blood, which is evidence of previous contact with this pathogen.
Just as with any other laboratory procedure, a definitive clinical diagnosis should not be based only on performing a test, but on the ensemble of a series of clinical and laboratory findings.
In the case of Ehrlichiosis, the presence of thrombocytopenia, leukopenia and anaemia are especially significant. These are laboratory findings which, along with performing the Ehrlichia test, confirm the disease.
How should I interpret a negative result with clear clinical symptoms of Ehrlichiosis?
If this is the case, we recommend that you verify the following points:
-If the test was performed with a very hemolysed blood, serum or plasma sample, or with lipemia, that may have interfered in the reaction.
– If a blood, serum or plasma sample that was kept cold and not brought up to appropriate room temperature was used.
– If the test was kept in the refrigerator and was not warmed up properly.
– If the aluminium envelope containing the test was open.
– If expired tests were used.
– If the test was not left in horizontal position before reading the results.
Our recommendation is:
1st Repeat the test, carefully following instructions to discard improper execution.
2nd Request or perform complementary tests (blood count, biochemical profile, etc.).
3rd Contact our technical assistance staff to report your case, analyse it and investigate possible causes.