Detection of antigen Dirofilariasis immitis in blood, serum or plasma.
Innovative one-step technique. 2 drops of sample are sufficient. No need to add reactive agents. Savings in time, fewer errors. Detects male and female parasites. Detects infestations with a load of only 1 adult parasite of any type (males, adult females, immature females, females that have not spread).
Formats available: 1 and 10 tests.
Technical documents only for veterinarians
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Frequently Asked Questions
What is the diagnostic interest of the Dirofilariasis Uranotest?
The detection of antigen Dirofilaria immitis in circulating blood.
Which sample provides the most reliable results: blood, serum or plasma?
Reliability is comparable when you use any of them.
However, when we work with very haemolysed or lipemic blood, it is always preferable to use serum or plasma, since haemolysis and lipemia are factors that interfere with any diagnostic technique, both at the clinic and at the Laboratory.
Can I dispense one drop of blood, serum or plasma directly from the syringe?
No. It is advisable to use the disposable pipette we provide and to dispense 3 drops on the sample pad.
Administering volume sample other than as recommended may lead to an erroneous result.
Can I use a tube other than the one included in the kit to collect blood?
Yes. You may use any other tube with anticoagulant treated with heparin, citrate or EDTA or with anticoagulant, if what you wish to do is use serum as a sample. The kit includes tubes with EDTA for your greater comfort and savings, but you may use any other one.
What should I do if I cannot use the blood at the time of its extraction?
You should proceed as if it were for any other laboratory procedure.
The entire blood must be kept refrigerated at between 2ºC and 7ºC for no more than 24 hours. It should never be frozen.
If you are not going to be able to use it before 24 hours, separate the plasma by centrifugation or the serum by sedimentation, and you may keep it at between 2ºC and 7ºC for up to 2 weeks. To preserve it for longer, freezing is recommended.
Can I use blood, serum or plasma immediately after removing it from the refrigerator?
No, as with any other diagnostic procedure, you must wait until it reaches room temperature. If want to speed this process up, hold the tube between your hands for at least 20 minutes. Many veterinarians do not take this circumstance into account, and incorrect temperature is one of the most frequent causes behind false results.
What happens if I forgot to do the reading at 5-10 minutes as recommended?
Between 5 and 10 minutes, you can read with total reliability.
20 minutes after doing the test, degradation and diffusion of the colours on the strip may occur. This does not happen with all tests, but the recommendation is that the test should always be repeated if the reading was not taken before the indicated time, since this may lead to an erroneous interpretation.
Do I not need to use a revealing buffer for the Dirofilaria Uranotest?
No. To make the technique even simpler, the Dirofilaria Uranotest was developed so that it can be carried out with no need for a revealing buffer.
By only depositing 3 drops of blood, serum or plasma on the round pad, migration begins immediately.
How should I interpret a positive result without clinical symptoms?
Screening or early diagnosis in preventative campaigns is one of the main applications for Dirofilaria Uranotests. As such, most of the time, a positive result will not be accompanied by clinical symptoms.
Dirofilaria Uranotest detects both male and female parasites, as of just one parasite of any type (males, adult females, immature females and non-propagated females), which makes this a very sensitive and useful technique for early disease diagnosis, including in very initial phases.
How should I interpret a negative result with clear evident of Dirofilariosis?
If this is the case, we recommend that you verify the following points:
-If the test was performed with a very haemolysed sample, or with lipemia, that may have interfered in the reaction.
– If a blood, serum or plasma sample that was kept cold and not brought up to appropriate room temperature was used.
– If the test was kept in the refrigerator and not brought up to room temperature before doing the test.
– If the aluminium envelope containing the test was open.
– If expired tests were used.
– If the test was not left in horizontal position before reading the results.
Our recommendation is:
1st Repeat the test, carefully following instructions to discard improper execution.
2nd Contact our technical assistance staff to report your case, analyse it and investigate possible causes.